Measurement of Complement Deficiencies
The complement system is a complex series of proteins that act as a cascade. They react in the sequence C1q, C1r, C1s, C4, C2, C3, C5, C6, C7, C8, C9; many requiring proteolytic cleavage to become active. The cascade is activated either via the Classical Pathway, the Alternative Pathway or the Lectin Pathway.
Complement is involved in initiating an inflammatory response and destroying certain bacteria and viruses. Complement proteins are assayed to measure complement consumption and to diagnose inherited deficiencies of cascade components.
Binding Site offers an extensive menu of complement assays for different platforms. Please see below for the complete menu.
The CH50 method is used as a screening test (for the measurement of total classical complement activity) when assessing the ability of an individual to fight infection.
The measurement of complement CH50 activity is recommended as part of the diagnostic protocol for Primary Immunodeficiency.1-2 It can also provide important information for many other disease states such as Systemic Lupus Erythematosus and bacterial infections.3
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Description
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Pack
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Code
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Classical Complement Pathway CH50 Eq ELISA kit
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96 test
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MK095
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| The Total Haemolytic Complement kit is primarily designed to detect deficiencies of the Classical Complement Pathway and the terminal sequence (C3-C9) components, whereas the Alternative Pathway Haemolytic Complement kit is designed to measure the activity of the Alternative Complement Pathway. |  |
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Description
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Pack
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Code
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Total Haemolytic
Complement kit
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3 plate kit
2 plate kit
1 plate kit |
RC001.3
RC001.2
RC001.1 |
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Alternative Pathway
Haemolytic Complement kit*
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3 plate kit
1 plate kit
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RC003.3*
RC003.1*
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*Research Use Only
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Description
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Pack
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Code
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C1 Inactivator RID kit – NL
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3 plate kit
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RN019.3
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C1q RID kit – NL+
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3 plate kit
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RN020.3
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C2 RID kit – NL
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3 plate kit
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RN022.3.U
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C3 RID kit – NL
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3 plate kit
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RN023.3
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| C3 MININEPH™ kit | 50 test | ZK023.R |
| C3 SPAPLUS kit | 100 test | NK023.R |
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C4 RID kit – NL
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3 plate kit
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RN025.3
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C4 - Binding Protein RID kit – NL*
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3 plate kit
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RN026.3*
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| C4 MININEPH™ kit | 50 test | ZK025.R |
| C4 SPAPLUS kit | 100 test | NK025.S |
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C5 RID kit – NL
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3 plate kit
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RN027.3
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C6 RID kit - NL*
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3 plate kit
1 plate kit
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RN102.3*
RN102.1*
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C7 RID kit - NL*
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3 plate kit
1 plate kit
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RN103.3*
RN103.1*
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C8 RID kit - NL*
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3 plate kit
1 plate kit
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RN089.3*
RN089.1*
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C9 RID kit – NL*
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3 plate kit
1 plate kit
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RN028.3*
RN028.1*
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Factor H (β 1H) RID kit - NL*
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3 plate kit
1 plate kit
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RN030.3*
RN030.1*
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Factor I RID kit - NL*
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1 plate kit
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RN031.1*
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+Diluted sample applied - assay range may be extended using undiluted sample.
*Research Use Only
Click here for additional RID kits, MININEPH™ kits, or SPAPLUS kits.
- De Vries E. Patient-centred screening for primary immunodeficiency: a multi-stage diagnostic protocol designed for non-immunologists. Clin Exp Immunol 2006;145:204-214
- Bonilla FA, et al. Practice parameter for the diagnosis and management of primary immunodeficiency. Ann Allergy Asthma Immunol 2005; 94:S1-63
- Botto M, et al. Complement in human diseases: Lessons from complement deficiencies. Mol Immunol 2009; 46:2774-2783
