Radial Immunodiffusion (RID) Kits
Thousands of laboratories world-wide rely upon a method of protein quantitation that has remained a "Gold Standard" for over 25 years. Radial immunodiffusion (RID), which is based on the complexing of antigen and antibody to produce a visible precipitin ring, is still a reference method to which new methods are compared.
RID remains a reliable laboratory diagnostic test method and is particularly useful for difficult samples e.g. that are turbid and for which other methods are inappropriate.
The BINDARID™ range of test kits, based on conventional RID technology, is suitable for measuring protein concentrations down to approximately 5mg/mL.
NANORID™ kits use a novel patented technology to enable accurate quantitation of very low concentrations of protein in serum, urine, cerebrospinal fluid and other biological materials. Sensitivities as low as 0.15 mg/mL can be achieved.
Binding Site has a wide range of radial immunodiffusion assays:
- Complement proteins including some functional assays
- Human immunoglobulins including IgG and IgA subclasses
- Coagulation
- Other human serum and plasma proteins
- And a Digital RID Plate Reader
Specifications
14 pre-cut wells per plate
Single high calibrator or calibration set (high, medium, low)
10 - fold measuring range
Plates readable by RID reference table (Mancini), Complete diffusion (Mancini) or Incomplete diffusion (Fahey and McKelvey)
RID assays for Human Complement Proteins
The complement system is a complex series of proteins that act as a cascade. They react in the sequence C1q, C1r, C1s, C4, C2, C3, C5, C6, C7, C8, C9; many requiring proteolytic cleavage to become active. The cascade is activated either via the Classical Pathway, the Alternative Pathway or the Lectin Pathway.
Complement is involved in initiating an inflammatory response and destroying certain bacteria and viruses. Complement proteins are assayed to measure complement consumption and to diagnose inherited deficiencies of cascade components.
Assays for Investigation of Complement Function
Total Haemolytic Complement (THC) and Alternative Pathway Haemolytic Complement (APHC) kits are effective screening assays to aid in the identification of complement deficiency.
The Total Haemolytic Complement kit is primarily designed to detect deficiencies of the Classical Complement Pathway and the terminal sequence (C3-C9) components, whereas the Alternative Pathway Haemolytic Complement kit is designed to measure the activity of the Alternative Complement Pathway.
- Simple methodology, no sample dilutions are necessary
- A 2 step incubation allows the formation of large, easy to read zones of haemolysis
- Results are interpreted easily without the need for expensive equipment
- Assays are supplied in kit format for ease of use
- 1, 2 or 3 plate kits are available to suit individual laboratory requirements
- A NEW Classical Complement Pathway CH50 Eq ELISA is also available
| Description | Pack | Code |
|---|---|---|
Total Haemolytic Complement kit
| 3 plate kit 2 plate kit 1 plate kit | RC001.3 RC001.2 RC001.1 |
Alternative Pathway Haemolytic Complement kit* | 3 plate kit 1 plate kit | RC003.3 RC003.1 |
C1 Inactivator Functional activity kit** C1 Inactivator Functional activity COMBI kit** Combi kit contains 2 functional activity plates and 1 antigenic quantitation plate. | 3 plate kit
3 plate kit | RC002.3
RK019 |
RID Assays for Investigation of Complement Deficiency
Binding Site also provides RID kits to detect and quantify individual components or factors in the complement cascade. 1 plate kits are available for a number of specificities, enabling small numbers of samples to be run economically.
| Description | Measuring Range (mg/L) | Pack | Code |
|---|---|---|---|
| C1 Inactivator - NL | 45 - 450 | 3 plate kit | RN019.3 |
| C1q - NL | 23 - 230# | 3 plate kit | RN020.3 |
| C2 - NL*** | 3.6 - 36 | 3 plate kit | RN022.3 |
| C3 - NL | 155 - 1550 | 3 plate kit | RN023.3 |
| C4 - NL | 58 - 580 | 3 plate kit | RN025.3 |
| C4 - Binding Protein - NL* | 50 - 500 | 3 plate kit | RN026.3 |
| C5 - NL | 20 - 200 | 3 plate kit | RN027.3 |
C6 - NL*
| 12 - 120
| 3 plate kit 1 plate kit | RN102.3 RN102.1 |
C7 - NL*
| 22 - 110
| 3 plate kit 1 plate kit | RN103.3 RN103.1 |
C8 - NL*
| 20 - 200 | 3 plate kit 1 plate kit | RN089.3 RN089.1 |
| C9 - NL | 50 - 500
| 3 plate kit 1 plate kit | RN028.3 RN028.1 |
| Factor B - NL** | 45 - 450 | 3 plate kit | RN029.3 |
Factor H (β 1H) - NL*
| 70 - 700
| 3 plate kit 1 plate kit | RN030.3 RN030.1 |
| Factor I - NL* | 7 - 70 | 1 plate kit | RN031.1 |
# Diluted sample applied - assay range may be extended using undiluted sample.
* Research use only
** Not available in the USA
*** In the USA for research use only
Human Immunoglobulin Kits
| Description | Measuring Range (mg/L) | Pack | Code |
|---|---|---|---|
| IgG - NL | 2250 - 22500 | 3 plate kit | RN004.3 |
| IgG - ML* | 18 - 180 | 3 plate kit | RL004.3 |
 - IgG - UL* | 0.45 - 4.5 | 3 plate kit | GL004.3 |
| IgG1 Subclass - SD | 1400 - 14000# | 3 plate kit | RN106.3 |
| IgG2 Subclass - SD | 800 - 8000# | 3 plate kit | RN107.3 |
| IgG3 Subclass - SD | 120 - 1200 | 3 plate kit | RN108.3 |
| IgG4 Subclass - SD | 50 - 500 | 3 plate kit | RN109.3 |
| IgG Subclass COMBI kit - SD | One plate each of IgG1, IgG2, IgG3 and IgG4 | 4 plate kit | RK021 |
| IgA - NL | 545 - 5450 | 3 plate kit | RN010.3 |
| IgA - ML* | 8.5 - 85 | 3 plate kit | RL010.3 |
| - IgA - UL* | 0.43 - 4.3 | 3 plate kit | GL010.3 |
| IgA Subclass COMBI kit - NL | Two plates each of IgA1 and IgA2 IgA1 640 - 6400 IgA2 50 - 500 | 4 plate kit | RK015 |
| IgM - NL | 265 - 2650 | 3 plate kit | RN012.3 |
| - IgM - ML* | 7 - 70 | 3 plate kit | GM012.3 |
| - IgM - UL* | 0.35 - 3.5 | 3 plate kit | GL012.3 |
| IgG, IgA, IgM COMBI kit - NL | One plate each of IgG, IgA and IgM | 3 plate kit | RK002 |
| IgD - NL | 5 - 50 mg/L | 3 plate kit | RN013.3 |
| Kappa - Free and Bound - NL* | 1500 - 15000 mg/L | 3 plate kit | RN015.3 |
| Kappa Free - EL* | 20 - 200 | 3 plate kit | RE016.3 |
| Lambda - Free and Bound - NL* | 825 - 8250# | 3 plate kit | RN017.3 |
| Lambda Free - EL* | 20 - 200 | 3 plate kit | RE018.3 |
# Diluted sample applied - assay range may be extended using undiluted sample.
Automated assays are also available for IgG and IgA Subclasses, IgD and free kappa and free lambda light chains.
Secretory IgA
There are two populations of IgA, a monomeric form in serum, and a dimeric form found in secretions such as saliva, tears, bronchial fluids etc. The dimeric form is covalently associated with a J-chain and a secretory piece. This secretory IgA (SIgA) represents the first line of defence against micro-organisms on the mucosal surface. SIgA deficiency results in a lack of immunity to mucosal viruses. This RID kit has been developed to specifically recognise only the SIgA population and not it's component forms (monomeric IgA, J-chain etc).
| Description | Measuring Range (mg/L) | Pack | Code |
|---|---|---|---|
| Secretory IgA - NL* | 45 - 450 | 3 plate kit | RN148.3 |
* Research use only
Human Coagulation Proteins
When a small blood vessel is injured, a series of reactions is initiated leading to the formation of a blood clot. This seals the vessel preventing further blood loss. Many of the clotting factors involved are enzymes which are activated during this cascade of events. There are three parts of the coagulation cascade; the intrinsic pathway, the extrinsic pathway and the common pathway. The end product of both the intrinsic, and the faster extrinsic pathway, is activated Factor X. This factor initiates a further cascade through the common pathway resulting in the formation of a stable fibrin clot at the site of injury.
Key factors in the clotting mechanism can be measured by Binding Site RID kits. These include Fibrinogen and Factor XIII, as well as protease inhibitors and anticoagulants that localise and limit the clotting process, for example anti-thrombin III (ATIII) and Protein C.
| Description | Measuring Range (mg/L) | Pack | Code |
|---|---|---|---|
| Antithrombin III - NL | 50 - 500 | 3 plate kit | RN040.3 |
| Factor XIII - NL* | 3 - 30# | 3 plate kit | GT113.3 |
| Fibrinogen - NL | 450 - 4500 | 3 plate kit | RN056.3 |
| Plasminogen - NL | 20 - 200 | 3 plate kit | RN065.3 |
| Protein C | 0.5 - 5.0 | 3 plate kit | GT118.3 |
# Diluted sample applied - assay range may be extended using undiluted sample.
* Research use only
Other Human Serum Protein RID kits
Radial immunodiffusion RID kits are available for laboratory detection of many other commonly measured human proteins, e.g. albumin.
| Description | Measuring Range (mg/L) | Pack | Code |
|---|---|---|---|
| Albumin - NL | 5000 - 50000# | 3 plate kit | RN032.3 |
| Albumin - ML* | 16 - 160 | 3 plate kit | RL032.3 |
| α1 - Antitrypsin - NL | 280 - 2800 | 3 plate kit | RN034 |
| β2 - Microglobulin - EL | 1 - 10 | 3 plate kit | GT043.3 |
| CRP - EL | 5.2 - 52 | 3 plate kit | GT044.3 |
| Caeruloplasmin - NL | 115 - 1150 | 3 plate kit | RN045.3 |
| Fibronectin - NL* | 50 - 500 | 3 plate kit | RN057.3 |
| Haptoglobin - NL | 50 - 500 | 3 plate kit | RN058.3 |
| Lysozyme - NL*** | 1.5 - 15 | 3 plate kit | GT073.3 |
| Prealbumin - NL (Transthyretin) | 43 - 430 | 3 plate kit | RN066.3 |
| Transferrin - NL | 440 - 4400 | 3 plate kit | RN070.3 |
# Diluted sample applied - assay range may be extended using undiluted sample.
* Research use only
*** In the USA for research use only
For animal RID kits please download the Immunobiological Reagents Product Listing.
Accessories
The jeweller's eyepiece facilitates measurement of precipitin rings to the nearest 0.1mm using a graduated scale visible through the x8 magnification eyepiece.
The disposable precision bore calibrated micropipettes with a stainless steel plunger assembly are ideal for accurate pipetting of 1 - 5 μl volumes.
| Description | Pack | Code |
|---|---|---|
| Digital RID Plate Reader | 1 | AD400 |
| Jeweller's Eye-Piece | 1 | AD040 |
1-5µL Micropipette plus 100 Disposable Capillaries | 1 | AD041 |
| RID Plates (unfilled) | 10 | IC003.1 |
BINDARID™ and NANORID™ are trademarks of The Binding Site Group Ltd., Birmingham, UK.
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