HEp-2 and HEp-2-AIM™
HEp-2 immunofluorescence assays are designed for the detection of anti-nuclear antibodies in human serum. These assays are intended as an aid in the screening and diagnosis of connective tissue diseases.
| Description | Well | Code |
|---|---|---|
HEp-2 IFA Kits
With Evans Blue in conjugate | 10 x 5 25 x 10 20 x 12 100 x 12
25 x 10 20 x 12 100 x 12 | FK001.1 FK001.2 FK001.6 FK001.7
FK001.2.E FK001.6.E FK001.7.E |
| Description | Well | Code |
|---|---|---|
| HEp-2 Slides | 10 x 5 25 x 10 100 x 10 20 x 12 100 x 12 10 x 24 100 x 24 | FS001.1 FS001.2 FS001.3 FS001.6 FS001.7 FS001.4 FS001.5 |
| Affinity purified anti human IgG (H&L), fluorescein conjugated (Prediluted for HEp-2) | 12.5mL 250 test | FA003.H |
A BINDAZYME™ ANA screening enzyme immunoassay is available.
Atlas of HEp-2 patterns Third Edition
A.R. Bradwell, R.G. Hughes
Atlas of HEp-2 patterns Third Edition
The book is available to order.
Please contact your local office or distributor
HEp-2 AIM™ - Rapid, simple, efficient
This optimised system simplifies HEp-2 methodology, whilst maintaining the accuracy and reproducibility of results.
AIM (Antibody Impregnated Membrane) technology comprises the coating of Fluorescein-conjugated antibody onto specialised paper strips. These are moistened and laid onto HEp-2 substrate slides enabling antibody/antigen reactions to occur.
Rapid - Incubation of conjugated antibody is only 10 minutes
Simple - No blotting prior to addition of conjugated antibody
Efficient - No dilution or dispensing of conjugated antibody required
Procedure Summary
1. IF-AIM™ paper strips are provided with the HEp-2 slides.
2. Diluted patient sera and controls are added to slide wells.
3. Slides are incubated at room temperature, then washed.
4. The IF-AIM™ paper strip is moistened with water.
5. Strips are laid onto the slides immediately. There is no need to blot the slide.
6. Slides are incubated for 10 minutes prior to washing.
7. Slides are blotted, mounted and viewed using fluorescence microscopy.
| Description | Well | Code |
|---|---|---|
HEp-2 IFA Kits incorporating AIM™ technology | 10 x 5 25 x 10 20 x 12 | FK001.1A FK001.2A FK001.6A |
| Description | Well | Code |
|---|---|---|
HEp-2 Slides incorporating AIM™ technology | 10 x 5 25 x 10 100 x 24 | FS001.1A FS001.2A FS001.5A |
- Download Literature - AIM™ Technology for Immunofluorescence - Code MKG285
- Download SPANISH Literature - AIM™ Technology for Immunofluorescence - Code MKG368
Return to list of Immunofluorescence assays (IFA)
AIM™ is a trademark of The Binding Site Ltd, Birmingham, UK
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