Conjugates / Controls
Fluorescein Conjugated Antibodies
Binding Site manufacture a range of Fluorescein labelled conjugates for use in IFA techniques. These are offered as affinity purified prediluted products or undiluted products. Prediluted conjugates are available with or without Evans Blue counterstain.
| Description | Pack | Code |
|---|---|---|
| Anti human IgG, A, M (Prediluted)* | 12.5mL/250 test | FA002 |
| Anti human IgG (H&L) (Prediluted) | 12.5mL/250 test | FA003 |
| Anti human IgG (γ chain) (Prediluted) | 12.5mL/250 test | FA004 |
| Anti human IgA (α chain) (Prediluted) | 12.5mL/250 test | FA010 |
| Anti human IgM (μ chain) (Prediluted)* | 12.5mL/250 test | FA012 |
| Anti human IgG, A, M with Evans Blue (Prediluted)* | 12.5mL/250 test | FA002.E |
| Anti human IgG (γ chain) with Evans Blue (Prediluted) | 12.5mL/250 test | FA004.E |
| Anti human IgA (α chain) with Evans Blue (Prediluted) | 12.5mL/250 test | FA010.E |
| Anti human IgM (μ chain) with Evans Blue (Prediluted)* | 12.5mL/250 test | FA012.E |
| Description | Pack | Code |
|---|---|---|
| Anti human IgG, A, M* | 1mL | AF002 |
| Anti human IgG (H&L)* | 1mL | AF003 |
| Anti human IgG (γ chain)** | 1mL | AF004 |
| Anti human IgA (α chain)* | 1mL | AF010 |
| Anti human IgM (μ chain)* | 1mL | AF012 |
* Not available in USA
** Analyte Specific Reagent in USA
These conjugates are supplied as 1mL undiluted. The information supplied with each product indicates approximate working titres for common applications. Ideally, every laboratory should establish the working titre on each batch of conjugate by chequerboard titration using their own immunofluorescence equipment.
Non-affinity purified Fluorescein conjugated antibodies are also available.
For non-rodent tissue
The use of non-rodent tissue may lead to high non-specific staining. This is easily addressed by the use of tissue specific conjugates which bind only to human immunoglobulin, for example in the detection of skin antibodies.
Comparison of non-species specific (left) and species-specific, anti-human IgG-Fluorescein (right) second antibodies.
Contact us for full information and a catalogue
Positive Control Sera
These control sera are prediluted, ready for use in immunofluorescence assay techniques. They are monospecific for the autoantigen stated. These controls are designed for use as 'working positive' control sera and the reactions they show are that of a moderate positive.
| Description | Pack | Code |
|---|---|---|
ANA - Homogenous ANA - Nucleolar ANA - Coarse Speckled C-ANCA P-ANCA Centromere dsDNA Endomysium IgA GAD (Glutamic Acid Decarboxylase)*** Gastric Parietal Cell Glomerular Basement Membrane (GBM) Hu (ANNA-1) Liver/Kidney Microsomal MAG (Myelin Associated Glycoprotein) Mitochondrial Negative Control Pancreatic Islet Cells*** Paraneoplastic Pemphigus Pemphigoid Pemphigus Smooth Muscle Steroidal Cell*** Striated Muscle Yo (PCA-1) | 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 1.0mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL | FA105 FA106 FA108 FA136 FA130 FA121 FA109 FA141 FA215 FA125 FA126 FA199 FA129 FA217 FA128 CON92 FA131 FA214 FA116 FA115 FA134 FA168 FA133 FA198 |
*** Check for availability
Positive Undiluted Control Sera
Sera may show positive reactions to more than one antigen.
| Description | Pack | Code |
|---|---|---|
C-ANCA (Proteinase 3) P-ANCA (Myeloperoxidase) Cardiolipin (IgG) Cardiolipin (IgM) dsDNA ENA - Jo-1 ENA - U1RNP ENA - Sm ENA - SS-A (Ro) ENA - SS-B (La) ENA - Scl-70 Endomysium (IgA) Gastric Parietal Cell Gliadin Glomerular Basement Membrane Hu (ANNA-1) Liver/Kidney Microsomal Mitochondrial Nuclear (ANA) - Centromere Nuclear (ANA) - Coarse Speckled Nuclear (ANA) - Homogeneous Nuclear (ANA) - Nucleolar Pancreatic Islet Cell Pemphigoid (Bullous) Pemphigus (Vulgaris) Reticulin 1 (IgA) Striated Muscle Smooth Muscle (SMA) Yo (PCA-1) | 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL 0.5mL | BP136 BP130 BP145 BP118 BP109 BP104 BP101 BP100 BP102 BP103 BP142 BP141 BP125 BP140 BP126 BP199 BP129 BP128 BP121 BP108 BP105 BP106 BP131 BP116 BP115 BP139 BP133 BP134 BP198 |
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