Classical Complement Pathway CH50 Eq ELISA
The CH50 method is used as a screening test (for the measurement of total classical complement activity) when assessing the ability of an individual to fight infection.
The measurement of complement CH50 activity is now recommended as part of the diagnostic protocol for Primary Immunodeficiency1,2 and it can also provide important information for many other disease states such as Systemic Lupus Erythematosus and bacterial infections.3
- Terminal Complement Complexes which occur naturally during sample handling are recognised by the CH50 Eq EIA
- No requirement for red blood cells and therefore not subject to seasonal variation and shelf-life limitations
- Good correlation with gold standard methods4
- Results are reported directly in CH50 Eq units by plotting a simple linear graph
- CH50 Eq EIA is provided as a complete kit which can be automated for ease of use
- Once activated, samples can be stored frozen at -20°C to allow batch testing
| Description | Pack | Code |
|---|---|---|
| Classical Complement Pathway CH50 Eq EIA kit Measuring range: 0-250 Eq U/mL | 96 test | MK095 |
Binding Site also supplies Radial Immunodiffusion assays for functional complement testing and quantitative assessment of complement components as well as MININEPH assays for C3, C4 and C1 inactivator.
References
- De Vries, E. Patient-centred screening for primary immunodeficiency: a multi-stage diagnostic protocol designed for non-immunologists. Clin Exp Immunol 2006;145(2):204-14
- Bonilla, F. A, Bernstein, I. L. et al. Practice parameter for the diagnosis and management of primary immunodeficiency. Ann Allergy Asthma Immunol 2005;94(5 Suppl 1):S1-63
- Botto, M, Kirschfink, M. et al. Complement in human diseases: Lessons from complement deficiencies. Mol Immunol 2009;46(14): 2774-83
- Kabat E. A. and Mayer M. M. Eds. Complement and Complement Fixation. Experimental Immunochemistry 1967 Springfield, IL.
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