Anti GBM IgG Kit
Anti GBM antibodies are useful as an aid in the diagnosis of Goodpasture's syndrome. This anti GBM EIA kit is designed for the quantitative measurement of specific IgG autoantibodies against the glomerular basement membrane (specifically the C-terminal end of type IV collagen) in human serum.
Contact us for information on detection of anti-GBM autoantibodies using an enhanced indirect immunofluorescence assay.
Antigen
Human recombinant Goodpasture antigen.
Calibration
The assay is calibrated in U/mL against an arbitrary reference calibrator, as no internationally recognised reference preparation is currently available. A 5 point calibration curve is generated. A cut-off control is provided if using as a screening assay.
Result Interpretation
Quantitative Assay
| Result | Interpretation |
|---|---|
| < 3.0 U/mL | Negative |
| > 3.0 U/mL | Positive |
Screening Assay
| Result | Interpretation | Action |
|---|---|---|
| OD equal to, or greater than cut-off control | Suspected of having anti-GBM antibodies | Test in the quantitative assay |
| OD less than cut-off control | Negative | Report as negative |
Measuring Range
1.23 - 100 U/mL
Sensitivity
0.1 U/mL
.jpg)