Anti Cardiolipin IgM S Kit

Autoantibodies against cardiolipin are found in a wide range of conditions such as systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). This anti cardiolipin EIA kit is designed for the detection and accurate quantitation of IgM class autoantibodies against cardiolipin in human serum samples.

Antigen

The microwells are precoated with purified bovine cardiolipin together with human and bovine ß2 glycoprotein-1 (cofactor).

Calibration

The assays are calibrated against the Sapporo EY2C9 monoclonal standard¹.

The Standardization Committee of the European Forum for Antiphospholipid Antibodies have supported the introduction of the Sapporo HCAL and EY2C9 IgG and IgM reference preparations. These standards are monoclonal antibodies that have dual functionality for both cardiolipin and β2GP-1 (results expressed in μg/mL). The monoclonal nature of these standard preparations ensures reproducibility^2-4.

A 5 point calibration curve is generated.

Result Interpretation

Result	Interpretation
< 1.40 μg/mL	Negative
> 1.40 μg/mL	Positive

Measuring Range

0.63 - 10 μg/mL

References

1. Ichikawa K, Khamashta M, Koike T, Matsuura E & Hughes GRV. (1994). β2-Glycoprotein I reactivity of monoclonal anticardiolipin antibodies from patients with the antiphospholipid syndrome. Arthritis Rheum; 37: 1453-61.

2. Fontain, M. et al. Persistent Problems with Standardization of Immunoassays for Anti-Cardiolipin Antibodies Thromb Haemost 2001; 86:1123-1124

3. Tincani, A. et al. Anticardiolipin Antibody Assay: a Methodological Analysis for a better Consensus in Routine Determinations. A Cooperative Project of the European Antiphospholipid Forum. Thromb Haemost 2001; 86 575-583

4. Ichikawa, K. et al. A chimeric antibody with the human y1 constant region as a putative standard for assays to detect IgG β2-glycoprotein 1-dependent anticardiolipin and anti-β2-glycoprotein 1 antibodies. Arthritis Rheum 1999; 42:2461-2470.

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